78 research outputs found
Infektivni peritonitis maÄaka (FIP) u naÅ”em sekcionom materijalu
Feline infectious peritonitis (FIP) has been diagnozed in our section material in 23 cats, 19 Persian and 4 domestic, of both sexes, aged between 5 months and 8 years. The majority of the infected population were animals under 20 months of age. The macroscopic finding in most cats was of granulomatous character, and large quantities of goldenyellow gelatinous exudate were observed in the stomach cavity of 7 animals, corresponding to the exudative form of FIP. Granulomas were in most cases located in the abdomen wall, liver, spleen, omentum and serous membrane of intestines, and the histological structure was characterized by fibrinoid-necrotic centers with numerous lymphocytes, monocytes, angioblasts and fibroblasts. Immunohistochemically, feline corona virus (FCV) antigens were exprimed in the cytoplasm of macrophages, more rarely in plasma cells in granulomas, and sometimes in necrotized areas.Infektivni peritonitis maÄaka (FIP) dijagnostikovan je u naÅ”em sekcionom materijalu kod 23 maÄke, 19 persijskih i 4 domaÄe, oba pola, uzrasta izmeÄu 5 meseci i 8 godina. VeÄinu inficirane populacije Äinile su životinje mlaÄe od 20 meseci. Makroskopski nalaz bio je kod veÄine maÄaka granulomatoznog karaktera, a kod 7 životinja uoÄena je veÄa koliÄina zlatno-žutog želatinoznog eksudata u trbuÅ”noj duplji, Å”to odgovara eksudativnoj formi FIP-a. Granulomi su bili smeÅ”teni u veÄini sluÄajeva u zidu abdomena, jetri slezini, omentumu i serozi creva, a histoloÅ”ka graÄa odlikovala se fibrinoidno-nekrotiÄnim centrima, sa mnogobrojnim limfocitima, monocitima angioblastima i fibroblastima. Imunohistohemijski, antigeni corona-virusa maÄaka (FCV) eksprimirani su u citoplazmi makrofaga, reÄe u plazma Äelijama u granulomima, a ponekad i u nekrotizovanim oblastima
Functional Outcome Assessment of Lower Limb Amputees and Prosthetic Users with a 2-Minute Walk Test
The aim of this study was to assess the functional outcome of a population of lower limb amputees supplied with prosthesis.
The research was conducted from June to September of 2010 at the Center for Physical and Rehabilitation Medicine,
of the Clinical Hospital Center Rijeka, Croatia. The study included 50 adult subjects of both genders with a unilateral
transtibial or transfemoral lower limb amputations. The 2-minute walk test (2MWT) was used to assess the functional
outcome of these individuals. Data were statistically analyzed. Subjects were divided into groups according to age. The
best results were obtained by subjects between the age of 45 and 59 years. The difference between groups was statistically
significant (p<0.001). Taking into account the cause of amputation, there was a statistically significant difference in the
results of the 2 MWT between subjects in whom the cause of amputation was circulatory and those where the cause of the
amputation was not due to circulatory problems. The best results were obtained in subjects in whom the cause of amputation
was not circulatory (p=0.009). Considering the level of amputation there was a statistically significant difference in
the results of the 2MWT between subjects with transtibial and those with transfemoral amputations. Better results were
obtained in transtibial amputees (p=0.039). Considering the first prosthetic supply, better results were obtained in subjects
using prosthetic devices over 9 years (p=0.031). Our research confirmed that age, gender, level and cause of amputation,
including the time from the first prosthetic supply have an effect on the 2MWT results
The role of hepatic myofibroblasts in liver cirrhosis in fallow deer (Dama dama) naturally infected with giant liver fluke (Fascioloides magna)
Background: This paper describes liver cirrhosis in 35 fallow deer infected with the giant liver fluke, as well as the distribution, origin, and role of myofibroblasts in its development. Results: In liver of infected deer, stripes of connective tissue are wound around groups of degenerated and regenerated liver lobuli. In the connective tissue, lymphocytes and macrophages which often contain parasite hematin are also present. The walls of the bile ducts are thickened, the epithelium multiplied with mucous metaplasia, and desquamated cells, parasite eggs and brown pigment are present in their lumen. In the livers with cirrhosis, immunopositivity to alpha-SMA and desmin was observed in cells in portal and septal spaces, at the edge between fibrotic septa and the surrounding parenchyma and in perisinusoidal spaces. These cells vary in size, they are round, oval, spindle-shaped or irregular in shape, similar to vascular smooth muscle cells. The derangement of epithelial-mesenchymal interactions detected in chronic cholangiopathies is most probably the pro-fibrogenic mechanism in liver cirrhosis of fallow deer (Dama dama) infected with the giant liver fluke (Fascioloides magna). Conclusion: Myofibroblasts, especially hepatic stellate cells (HSCs), play an important role in the synthesis of extracellular matrix components in the development of parasitic fibrosis and cirrhosis in the liver of fallow deer
SOWING RATE INFLUENCE IN SPRING BARLEY GRAIN YIELD AND ITS COMPONENTS IN SLAVONIA AND BARANIA REGION
Conjunctival Amelanotic Melanoma ā A Case Report
Conjunctival melanoma is a relatively rare malignancy. It is presented as pigmented nodule in any area of conjunctiva,
amelanotic tumors are pink with smooth apperance. The authors describe an amelanotic melanoma of the conjunctiva
in an 82-year-old female patient. Cytological, histopathological and immunohistochemical studies revealed an invasive
amelanotic melanoma exhibiting S-100 and MART-1 positivity. The patient undervent surgical and chemotherapy
treatement and three years after the initial treatment is in the terminal stage of metastatic disease. Absence of pigmentation
delayed early clinical detection and treatement. Awareness of this nonpigmented melanoma is crucial for early recognition
and appropriate management
Prognostic markers significant for therapy of tumours in dogs
ViÅ”estruki onkogeni efekti utiÄu na inhibiciju maturacije Äelije, migraciju i tkivnu invaziju. Razvoj tumora podrazumeva nesklad izmeÄu proliferacije, rasta i diferencijacije, Å”to je praÄeno znaÄajnim genotipskim i fenotipskim varijacijama. Kvalitativno i kvantitativno odreÄivanje bioloÅ”kih prognostiÄkih faktora u tkivu govori o metastatskom potencijalu tumora i predikciji neoplastiÄne bolesti. Faktore angiogeneze i brojne aktivirajuÄe i inhibitorne proteine moguÄe je imunohistohemijski dokazati u tkivu tumora, a imunofenotipizacija limfoma je nužna za izbor terapijskog protokola. OdreÄivanje proliferativnog potencijala tumorskih Äelija imunohistohemijskom detekcijom PCNA, Ki-67, CD-117, CD45 koristi se u gradiranju mastocitoma. Sve je znaÄajnija kliniÄka korelacija rasta tumora sa ekspresijom razliÄitih antigena na tumorskim Äelijama.The multiple oncogenic effects on the inhibition of cell maturation, on cell migration and tissue invasion,
is responsible for the occurrence of tumours. The development of tumours implies a disproportion between
proliferation, growth and differentiation, which is accompanied by significant genotypic and phenotypic
variations in the diseased. The qualitative and quantitative determination of the biological prognostic factors
in the tissue indicates possible tumour metastasizing, and consequently also the prognosis of the neoplastic
disease. It is possible immunohistochemically to prove factors of angiogenesis and numerous activating and
inhibiting proteins in the tumour tissue, which is also very important for properly choice of lymphoma
therapy. The determination of the proliferative potential of tumour cells by immunohistochemical detection of
PCNA, Ki-67, CD-117, CD 45, is used in grading of mast cell tumours. In addition to other histological,
clinical and biological parameters, they are significant for the further prognosis and therapy of neoplasms
Mastocitom unutar lipoma kod bele toj pudle
Uprkos Äinjenici da su mastocitomi jedna od najÄeÅ”cĢih neoplazmi kod pasa, postoji samo jedan objavljeni sluÄaj mastocitoma u lipomu. U ovom sluÄaju, kod 11-godiÅ”nje ženke toj pudle primecĢeno je prisustvo dobro ograniÄene, pokretne, meko-elastiÄne mase na lateralnoj strani grudnog koÅ”a. HistopatoloÅ”ki pregled je pokazao dobro ograniÄenu masu koja se sastojala od zrelih adipocita izmeÄu kojih se nalaze skupine cĢelija sa granuliranom citoplazmom. Bojenjem toluidin plavim je dokazano prisustvo metahromatskih granula u citoplazmi mastocita. HistopatoloÅ”ke i imunohistohemijske karakteristike ukazale su na to da je ispitani mastocitom niskog stepena maligniteta
MorfoloÅ”ko i imunocitohemijsko ispitivanje tumorskih Äelija kod Marekove bolesti
In this paper are described the macroscopic, microscopic and immunophenotypic characteristics of tumors in Marekās disease (MD). Liver, kidneys, lungs, heart, proventriclus, ovary and nerves of sciatic plexus collected from 20 chickens aged 8 to 24 weeks were examined. Lymphoproliferative enlargement in various organs of focal and diffuse character was found. The affected organs were enlarged, compact and very brittle, irregular in shape, grayish-red or grayish-white and of fatty consistency. Most often characteristic changes were in the spleen, liver, proventriculus and ovary that usually had a compact or ribbed, fatty cauliflower like formation, different in size. On histological examination proliferation of small and medium size lymphocytes, lymphoblast cells, Marekās disease cells and activated reticulum cells were found. Tumor proliferates predominantly consisted of lymphoblasts and had all the characteristics of a lymphoma. Immunohistochemical analysis revealed that lymphoma cells in MD are of CD3 + phenotype. In liver proliferates numerous CD79+ cells were found perivasculary, which is an interesting finding demanding further research.U ovom radu su opisane makroskopske, mikroskopske i imunofenotipske karakteristike tumora kod Marekove bolesti (MB). Ispitivanjem su bili obuhvaÄeni jetra, bubreg, pluÄa, srce, žlezdani želudac, jajnik i nervi ishijadiÄnog pleksusa dvadeset piliÄa starosti izmeÄu8i24 nedelje. U ispitivanom materijalu ustanovljeno je limfoproliferativno bujanje u razliÄitim organima, fokalnog ili difuznog karaktera, pri Äemu su zahvaÄeni organi znatno poveÄani, kompaktni i veoma krti, Äesto nepravilnog oblika, sivocrveni ili sivobeli, slaninastog izgleda. NajÄeÅ”Äe i najkarakteristiÄnije su bili promenjeni slezina, jetra, žlezdani želudac, jajnici koji obiÄno predstavljaju kompaktne ili izbrazdane tvorevine poput karfiola, slaninastog izgleda i razliÄite veliÄine. HistoloÅ”ki, u organima se nalaze proliferati malih i srednjih limfocita, zatim limfoblasta, Äelija Marekove bolesti i aktivisane retikulumske Äelije. Tumorski proliferati su predominantno sastavljeni od limfoblasta i imaju sve karakteristike limfoma. Imunohistohemijskom analizom je ustanovljeno da su Äelije limfoma kod MB predominantno T Äelijskog tipa, CD3 fenotipa. U proliferatu u jetri su naÄene i brojne CD79 pozitivne Äelije lokalizovane perivaskularno, Å”to predstavlja interesantan nalaz i zahteva dalja ispitivanja
Ekspresija KIT receptora na kutanim mastocitomima pasa bez c-kit mutacija
Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation.HistopatoloÅ”ko ispitivanje, gradacija, imunohistohemijsko bojenje i molekularno genetska ispitivanja su predloženi kriterijumi za klasifikaciju kutanih mastocitoma pasa. Prisustvo aberantne ekspresije CD117 i mutacija c-kit protoonkogena moglo bi da bude indikativni parameter za finalnu histopatoloÅ”ku klasifikaciju mastocitoma. Glavni cilj ispitivanja je odreÄivanje povezanosti mesta ekspresije KIT receptora i histoloÅ”kog gradusa mastocitoma bez c-kit mutacija. Ispitivanjima su obuhvaÄena 24 mastocitoma i 6 kontrolnih iseÄaka kože uzorkovanih od ukupno 30 odraslih pasa razliÄitog uzrasta, rase i pola. Uzorci fiksirani u formalinu i ukalupljeni u parafinske kalupe su bojeni hematoksilin-eozinom, toluidin plavo metodom i imunohistohemijski primenom CD117 antitela. DNK je izolovana iz istih parafinskih kalupa, a PCR amplifikacija je izvrÅ”ena primenom specifiÄnih prajmera PE1 i PE2. Stepen malignosti je utvrÄen na osnovu prisustva mitotskih figura, multinuklearnih Äelija, Äelija sa bizarnim jedrom i kariomegalije na 10 polja velikog uveliÄanja. Na osnovu histopatoloÅ”kih osobina, 14 od 24 mastocitoma su svrstani u visoki stepen maligniteta, dok je 10 mastocitoma niskog stepena maligniteta. Citoplazmatska ekspresija CD117 je uoÄena kod 9 od 14 mastocitoma visokog stepena maligniteta, Å”to potvrÄuje vezu izmeÄu aberantne ekspresije CD117 i poveÄane Äelijske proliferacije
Efikasnost detoksikujuÄeg agensa kod T-2 toksikoze brojlera
This investigation was conducted in order to investigate the efficacy of the detoxifying agent MycofixĀ® Plus (MP) in the prevention and/or alleviation in vivo adverse effects of T-2 toxin in broilers. In addition, the adsorbing potential of MP was estimated in vitro. Mean degradation levels of T-2 toxin with MP in vitro, as measured by HPTLC, varied from 26.06 to 31.02 % and the adsorption ability was elevated in acidic environment (pH 3). In vivo trial was performed on 160 one day old 'Ross' broiler chicks and lasted for 21 days. Birds were divided into 4 equal groups as follows: Group 1 - negative control; Group 2 - positive control - 2 ppm T-2 toxin; Group 3 - 2 ppm T-2 toxin+2 kg/t MP; Group 4 - 2 kg/t MP. Broilers fed the diet containing 2 mg/kg of T-2 toxin without MP developed typical T-2 toxicosis. Birds that were fed the diet containing both T-2 and MP had better performances and no oral ulcerations as the dominant sign of T-2 toxicosis were observed. Histopathological examination of tissues originating from birds fed the diet containing T-2 toxin revealed degenerative changes in the oral and small intestine mucosa, necroses of enterocytes and hepatocytes, as well as depletion of lymphocytes in the bursa Fabricii. Immunohistochemical examination also revealed negative effects of T-2 toxin on cells proliferation in intestineal and bile duct mucosa, as well as on lymphocytes from bursa Fabricii. The macroscopic and microscopic structure of the liver, intestine and bursa Fabricii of broilers fed a diet containing T-2 toxin and MP was mostly preserved. Cutaneous basophile hypersensitivity reaction was weaker in broilers fed mixtures containing 2 mg/kg T-2 toxin.U cilju ispitivanja efikasnosti detoksikujuÄeg agensa MycofixĀ® Plus (MP) na preveniranje ili ublažavanje Å”tetnih efekata T-2 toksikoze kod brojlera izvedena su ispitivanja u in vitro uslovima, kao i in vivo ogled. ProseÄne vrednosti degradacije T-2 toksina od strane MP u in vitro uslovima, ustanovljene HPTLC metodom, su iznosile 26,06 do 31,02 %, pri Äemu je konstatovana veÄa sposobnost adsorpcije ovog toksina u kiseloj sredini (pH 3). In vivo ogled je bio postavljen na 160 jednodnevnih brojlera provenijencije 'Ross', podeljenih u 4 eksperimentalne grupe sa po 40 jedinki u svakoj i trajao je 21 dan. PraÄenjem zdravstvenog stanja, kliniÄke slike i proizvodnih rezultata, kod brojlera koji su u hrani dobijali umeÅ”an T-2 toksin u koliÄini od 2 mg/kg, konstatovani su karakteristiÄni simptomi za T-2 toksikozu. Kod brojlera koji su uz toksin, hranom dobijali iMP, konstatovani su zaÅ”titni efekti u vidu izostanka ulceracija u usnoj duplji i boljih proizvodnih rezultata u odnosu na brojlere koji su dobijali samo T-2 toksin. PatohistoloÅ”kim ispitivanjima uoÄeni su negativni efekti T-2 toksina u svim ispitivanim organima u vidu degenerativnih promena na sluzokoži usne duplje i tankog creva, nekroza enterocita i hepatocita kao i deplecija limfocita u Fabricijevoj burzi. Imunohistohemijskim ispitivanjima uoÄeni su negativni efekti T-2 toksina na proliferativnu aktivnost Äelija mukoze creva, žuÄnih kanala i limfocita u Fabricijevoj burzi. Kod brojlera koji su putem hrane dobijali T-2 toksin i adsorbens MP, zapaža se uglavnom oÄuvana makroskopska i mikroskopska struktura jetre, creva i Fabricijeve burze. Reakcija kožne preosetljivosti na PHA je bila slabija kod brojlera koji su hranom dobijali samo T-2 toksin
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